http://en.wikipedia.org/wiki/Escherichia_coli
The interactome of E. coli has been studied by affinity purification and mass spectrometry (AP/MS) and by analyzing the binary interactions among its proteins.
Protein complexes. A 2006 study purified 4,339 proteins from cultures of strain K-12 and found interacting partners for 2,667 proteins, many of which had unknown functions at the time.A 2009 study found 5,993 interactions between proteins of the same E. coli strain though this data showed little overlap with that of the 2006 publication.
Binary interactions. Rajagopala et al. (2014) have carried out systematic yeast two-hybrid screens with most E. coli proteins and found a total of 2,234 protein-protein interactions.This study also integrated genetic interactions and protein structures and mapped 458 interactions within 227 protein complexes.
The interactome of E. coli has been studied by affinity purification and mass spectrometry (AP/MS) and by analyzing the binary interactions among its proteins.
Protein complexes. A 2006 study purified 4,339 proteins from cultures of strain K-12 and found interacting partners for 2,667 proteins, many of which had unknown functions at the time.A 2009 study found 5,993 interactions between proteins of the same E. coli strain though this data showed little overlap with that of the 2006 publication.
Binary interactions. Rajagopala et al. (2014) have carried out systematic yeast two-hybrid screens with most E. coli proteins and found a total of 2,234 protein-protein interactions.This study also integrated genetic interactions and protein structures and mapped 458 interactions within 227 protein complexes.
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